The affected person gargles the answer and rinses it contained in the tube.
The National Environmental Engineering Research Institute (NEERI) has developed a easy and fast method of swab collection and processing for RT-PCR coronavirus check which may very well be utilized in rural and tribal areas.
The method is easy, fast, cost-effective, patient-friendly and snug, the Council for Scientific and Industrial Research (CSIR) mentioned.
It is well-suited for rural and tribal areas, given minimal infrastructure necessities, it mentioned.
The Nagpur-based NEERI is a constituent laboratory of CSIR.
Krishna Khairnar, senior scientist, Environmental Virology Cell at NEERI, mentioned the swab collection method requires time. Moreover, since it’s an invasive approach, it’s a bit uncomfortable for sufferers.
“Sometime, it is also lost in the transport of the sample to the collection centre. On the other hand, the Saline Gargle RT-PCR method is instant, comfortable and patient-friendly. Sampling is done instantly and results will be generated within three hours,” he mentioned.
The method is non-invasive and so easy {that a} affected person can accumulate the pattern himself, mentioned Mr. Khairnar.
Collection strategies like nasopharyngeal and oropharyngeal swab collection require technical experience and they’re additionally time-consuming. In distinction, the Saline Gargle RT-PCR method makes use of a easy collection tube full of saline resolution, he mentioned.
The affected person gargles the answer and rinses it contained in the tube. This pattern within the collection tube is taken to the laboratory the place it’s saved at room temperature, in a particular buffer resolution ready by NEERI.
An RNA template is produced when this resolution is heated, which is additional processed for Reverse Transcription-Polymerase Chain Reaction (RT-PCR).
This specific method of accumulating and processing the pattern permits to avoid wasting on the in any other case expensive infrastructural requirement of RNA extraction. The method is environment-friendly as effectively, since waste technology is minimised, Mr. Khairnar mentioned.
The Nagpur Municipal Corporation has given permission to go forward with the method, following which testing has begun at NEERI, the CSIR mentioned.
